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Molecular and biological characterization of a recombinant isolate of potato virus Y from Mexico

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Abstract

An isolate of potato virus Y (PVY), PVY-M3, was subjected to biological characterization on potato indicators and to whole-genome sequencing. PVY-M3 induced a local and systemic hypersensitive resistance (HR) response in potato cultivar Maris Bard expressing the Nz gene while inducing no HR in potato cultivars Desiree and King Edward, carrying Ny and Nc genes, respectively. These HR responses, combined with a lack of vein necrosis in tobacco, clearly defined PVY-M3 as an isolate of the PVYZ strain. Recombination analysis demonstrated that PVY-M3 had a typical European PVYNTN genome with three recombinant junctions, and PVYN and PVYO were identified as parents.

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References

  1. Beczner L, Horvath J, Romhanyi I, Forster H (1984) Studies on the etiology of tuber necrotic ringspot disease in potato. Potato Res 27:339–352

    Article  Google Scholar 

  2. Blanchard A, Rolland M, Lacroix C, Kerlan C, Jacquot J (2008) Potato virus Y: a century of evolution. Curr Topics Virol 7:21–32

    CAS  Google Scholar 

  3. Boonham N, Walsh K, Hims M, Preston S, North J, Barker I (2002) Biological and sequence comparisons of Potato virus Y isolates associated with potato tuber necrotic ringspot disease. Plant Pathol 51:117–126

    Article  CAS  Google Scholar 

  4. Chikh Ali M, Maoka T, Natsuaki KT (2007) The occurrence and characterization of new recombinant isolates of PVY displaying shared properties of PVYNW and PVYNTN. J Phytopathol 155:409–415

    Article  Google Scholar 

  5. Chikh Ali M, Maoka T, Natsuaki T, Natsuaki KT (2010) PVYNTN-NW, a novel recombinant strain of Potato virus Y predominating in potato fields in Syria. Plant Pathol 59:31–41

    Article  Google Scholar 

  6. Chikh Ali M, Maoka T, Natsuaki T, Karasev AV (2012) A new recombinant Potato virus Y isolate classified as belonging to PVYZ strain group may help to define viral determinant responsible for tuber necrosis in potato. Phytopathology 102:S.4.23

    Google Scholar 

  7. Cockerham G (1970) Genetical studies on resistance to potato viruses X and Y. Heredity 25:309–348

    Article  Google Scholar 

  8. Crosslin JM, Hamm PB, Eastwell KC, Thornton RE, Brown CR, Corsini D, Shiel JP, Berger PH (2002) First report of necrotic strain of Potato virus Y (PVYN) on potatoes in the Northwestern United States. Plant Dis 86:1177

    Article  Google Scholar 

  9. de Bokx J A, Huttinga H (1981) Potato virus Y. Descriptions of plant viruses, No. 242. Commonw Mycol Inst/Assoc Appl Biol, Kew, England. www.dpvweb.net/dpv/showdpv.php?dpvno=242

  10. Djilani-Khouadja F, Glais L, Tribodet M, Kerlan C, Fakhfakh H (2010) Incidence of potato viruses and characterization of Potato virus Y variability in late season planted potato crops in Northern Tunisia. Eur J Plant Pathol 126:479–488

    Article  CAS  Google Scholar 

  11. Dullemans AM, Cuperus C, Verbeek M, van der Vlugt RA (2011) Complete nucleotide sequence of a potato isolate of strain group C of Potato virus Y from 1938. Arch Virol 156:473–477

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. Galvino-Costa SBF, Figueira A, Camargos VV, Geraldino PS, Hu X, Nikolaeva OV, Kerlan C, Karasev AV (2012) A novel type of Potato virus Y recombinant genome, determined for the genetic strain PVYE. Plant Pathol 61:388–398

    Article  CAS  Google Scholar 

  13. Galvino-Costa SBF, Figueira AR, Rabelo-Filho FAC, Moraes FHR, Nikolaeva OV, Karasev AV (2012) Molecular typing of Potato virus Y isolates from Brazil reveals a diverse set of recombinant strains. Plant Dis 96:1451–1458

    Article  CAS  Google Scholar 

  14. Glais L, Tribodet M, Gauthier JP, Astier-Manifacier S, Robaglia C, Kerlan C (1998) RFLP mapping of the whole genome of ten viral isolates representative of different biological groups of Potato virus Y. Arch Virol 143:2077–2091

    Article  CAS  PubMed  Google Scholar 

  15. Glais L, Tribodet M, Kerlan C (2002) Genomic variability in Potato potyvirus Y (PVY): evidence that PVYNW and PVYNTN variants are single or multiple recombinants between PVYO and PVYN isolates. Arch Virol 147:363–378

    Article  CAS  PubMed  Google Scholar 

  16. Gray SM, DeBoer SH, Lorenzen J, Karasev AV, Whitworth J, Nolte P, Singh RP, Boucher A, Xu H (2010) Potato virus Y: a significant and evolving threat to potato crops in the United States and Canada. Plant Dis 94:1384–1397

    Article  Google Scholar 

  17. Hu X, Meacham T, Ewing L, Gray SM, Karasev AV (2009) A novel recombinant strain of Potato virus Y suggests a new viral genetic determinant of vein necrosis in tobacco. Virus Res 143:68–76

    Article  CAS  PubMed  Google Scholar 

  18. Jones RAC (1990) Strain group specific and virus specific hypersensitive reactions to infection with potyviruses in potato cultivars. Ann Appl Biol 117:93–105

    Article  Google Scholar 

  19. Karasev AV, Gray SM (2013) Continuous and emerging challenges of Potato virus Y in potato. Annu Rev Phytopathol 51:571–586

    Article  CAS  PubMed  Google Scholar 

  20. Karasev AV, Hu X, Brown CJ, Kerlan C, Nikolaeva OV, Crosslin JM, Gray SM (2011) Genetic diversity of the ordinary strain of Potato virus Y (PVY) and origin of recombinant PVY strains. Phytopathology 101:778–785

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Karasev AV, Nikolaeva OV, Hu X, Sielaff Z, Whitworth J, Lorenzen JH, Gray SM (2010) Serological properties of ordinary and necrotic isolates of Potato virus Y: a case study of PVYN misidentification. Am J Potato Res 87:1–9

    Article  Google Scholar 

  22. Kerlan C (2006) Description of plant viruses: Potato virus Y. Association of applied biologists. [http://www.dpvweb.net/dpv/showdpv.php?dpvno=414]

  23. Kerlan C, Nikolaeva OV, Hu X, Meacham T, Gray SM, Karasev AV (2011) Identification of the molecular make-up of the Potato virus Y strain PVYZ: genetic typing of PVYZ-NTN. Phytopathology 101:1052–1060

    Article  CAS  PubMed  Google Scholar 

  24. Kerlan C, Tribodet M, Glais L, Guillet M (1999) Variability of Potato virus Y in potato crops in France. J Phytopathol 147:643–651

    Article  Google Scholar 

  25. Le Romancer M, Kerlan C, Nedellec M (1994) Biological characterization of various geographical isolates of potato virus Y inducing superficial necrosis on potato tubers. Plant Pathol 43:138–144

    Article  Google Scholar 

  26. Lorenzen JH, Meacham T, Berger PH, Shiel PJ, Crosslin JM, Hamm PB, Kopp H (2006) Whole genome characterization of Potato virus Y isolates collected in the western USA and their comparison to isolates from Europe and Canada. Arch Virol 151:1055–1074

    Article  CAS  PubMed  Google Scholar 

  27. Lorenzen JH, Piche LM, Gudmestad NC, Meacham T, Shiel P (2006) A multiplex PCR assay to characterize Potato virus Y isolates and identify strain mixtures. Plant Dis 90:935–940

    Article  CAS  Google Scholar 

  28. Lorenzen JH, Nolte P, Martin D, Pasche JS, Gudmestad NC (2008) NE-11 represents a new strain variant class of Potato virus Y. Arch Virol 153:517–525

    Article  CAS  PubMed  Google Scholar 

  29. McDonald JG, Kristjansson GT (1993) Properties of strains of Potato virus YN in North America. Plant Dis 77:87–89

    Article  Google Scholar 

  30. McDonald JG, Singh RP (1993) Assessment of North American isolates of PVYN for strains that induce tuber necrotic ring necrosis disease. Am Potato J 70:827

    Google Scholar 

  31. Nikolaeva OV, Roop D, Galvino-Costa SFB, Figueira AR, Gray SM, Karasev AV (2012) Epitope mapping for monoclonal antibodies recognizing tuber necrotic strains of Potato virus Y. Am J Potato Res 89:121–128

    Article  CAS  Google Scholar 

  32. Nolte P, Whitworth JL, Thomton MK, McIntosh CS (2004) Effect of seedborne Potato virus Y on performance of Russet Burbank, Russet Norkotah and Shepody potato. Plant Dis 88:248–252

    Article  Google Scholar 

  33. Piche LM, Singh RP, Nie X, Gudmestad NC (2004) Diversity among Potato virus Y isolates obtained from potatoes grown in the United States. Phytopathology 94:1368–1375

    Article  CAS  PubMed  Google Scholar 

  34. Quintero-Ferrer A, Karasev AV (2013) First report of recombinant Potato virus Y strains in potato in Jalisco, Mexico. Plant Dis 97:430

    Article  Google Scholar 

  35. Ramírez-Rodríguez VR, Aviña-Padilla K, Frías-Treviño G, Martínez-Soriano JP (2009) Presence of necrotic strains of Potato virus Y in Mexican potatoes. Virol J 6:80. doi:10.1186/1743-422X-6-80

    Article  Google Scholar 

  36. Robles-Hernández L, González-Franco AC, Hernández-Huerta J, Meacham TM, Nikolaeva OV, Karasev AV (2010) First identification of an unusual recombinant Potato virus Y strain in potato in Mexico. Plant Dis 94:1262

    Article  Google Scholar 

  37. Schubert J, Fomitcheva V, Sztangret-Wisniewska J (2007) Differentiation of Potato virus Y strains using improved sets of diagnostic PCR-primers. J Virol Methods 140:66–74

    Article  CAS  PubMed  Google Scholar 

  38. Singh RP, Valkonen JPT, Gray SM, Boonham N, Jones RAC, Kerlan C, Schubert J (2008) Discussion paper: the naming of Potato virus Y strains infecting potato. Arch Virol 153:1–13

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors would like to thank Teresa Meacham and Kelsie Evans for help in sequencing and data analysis. This work was supported in part through grants from USDA-NIFA-NRI (2009-35600-05025), USDA-NIFA-SCRI (2009-51181-05894), the USDA-ARS Cooperative Agreement 58-5354-7-540, the U.S. Potato Board, and the Idaho Potato Commission.

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Correspondence to Alexander V. Karasev.

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Supplementary Figure: A cladogram of nucleotide sequences of potato virus Y (PVY) isolates. The phylogenetic trees were constructed based on whole genomes of each isolate. One hundred bootstrap replicates were analyzed by the maximum-likelihood algorithm, and the figure shows the 70 % consensus tree. Bootstrap support for each node is shown. Brackets indicate the main recombinant and non-recombinant types of PVY.

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Quintero-Ferrer, A., Robles-Hernandez, L., Gonzalez-Franco, A.C. et al. Molecular and biological characterization of a recombinant isolate of potato virus Y from Mexico. Arch Virol 159, 1781–1785 (2014). https://doi.org/10.1007/s00705-013-1968-0

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  • DOI: https://doi.org/10.1007/s00705-013-1968-0

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