Abstract
An isolate of potato virus Y (PVY), PVY-M3, was subjected to biological characterization on potato indicators and to whole-genome sequencing. PVY-M3 induced a local and systemic hypersensitive resistance (HR) response in potato cultivar Maris Bard expressing the Nz gene while inducing no HR in potato cultivars Desiree and King Edward, carrying Ny and Nc genes, respectively. These HR responses, combined with a lack of vein necrosis in tobacco, clearly defined PVY-M3 as an isolate of the PVYZ strain. Recombination analysis demonstrated that PVY-M3 had a typical European PVYNTN genome with three recombinant junctions, and PVYN and PVYO were identified as parents.
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Acknowledgments
The authors would like to thank Teresa Meacham and Kelsie Evans for help in sequencing and data analysis. This work was supported in part through grants from USDA-NIFA-NRI (2009-35600-05025), USDA-NIFA-SCRI (2009-51181-05894), the USDA-ARS Cooperative Agreement 58-5354-7-540, the U.S. Potato Board, and the Idaho Potato Commission.
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Supplementary Figure: A cladogram of nucleotide sequences of potato virus Y (PVY) isolates. The phylogenetic trees were constructed based on whole genomes of each isolate. One hundred bootstrap replicates were analyzed by the maximum-likelihood algorithm, and the figure shows the 70 % consensus tree. Bootstrap support for each node is shown. Brackets indicate the main recombinant and non-recombinant types of PVY.
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Quintero-Ferrer, A., Robles-Hernandez, L., Gonzalez-Franco, A.C. et al. Molecular and biological characterization of a recombinant isolate of potato virus Y from Mexico. Arch Virol 159, 1781–1785 (2014). https://doi.org/10.1007/s00705-013-1968-0
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DOI: https://doi.org/10.1007/s00705-013-1968-0